A reservoir-type oxygen sensor with 2×3 array for measuring cellular respiration levels

  • Park J
  • Nam H
  • Ahn S
 et al. 
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This paper describes a 2 × 3 reservoir-type oxygen sensor array integrated with Clark-type sensors, heaters, and temperature sensors on a glass substrate. The measured 90% response time of the fabricated sensors from an air-saturated state to a zero-oxygen state was approximately 9.35 s. These oxygen sensors showed good repeatability during 6 consecutive measurements at 300-s intervals, with an output of 108.2 ± 3.15 nA (mean ± standard deviation) for the air-saturated state and 1.33 ± 0.14 nA for the zerooxygen state. In addition, these sensors showed good linearity, with a correlation coefficient of 0.995 and a sensitivity averaging 2.04 A/cm2 M. A LabVIEW-based measurement system was developed for simultaneously measuring the multiple cellular respiration levels from this oxygen sensor array using a voltage supply, an amplifying module, a DMM (digital multimeter), a switch module, and a temperature-sensing module. The measured cellular respiration ratio of the L6 (rat skeletal muscle cells) between the uncoupled oxygen consumption rate and the coupled oxygen consumption rate for this oxygen sensor array was 2.06, which is similar to the 2.23 value obtained from a commercially available system. © 2012 Elsevier B.V. All rights reserved.

Author-supplied keywords

  • Array type
  • Cellular respiration
  • Electrochemistry
  • Oxygen consumption rate
  • Oxygen sensor

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