Residual dipolar couplings and some specific models for motional averaging

  • Deschamps M
  • Campbell I
  • Boyd J
  • 20

    Readers

    Mendeley users who have this article in their library.
  • 14

    Citations

    Citations of this article.

Abstract

The measurement of residual dipolar couplings (RDCs) from partially oriented molecules is now widely used to provide restraints for NMR structure determination. Bond vibrations, random angular fluctuations around bond vectors and conformational exchange all influence the magnitude of the experimental RDCs. The effect that angular fluctuations have upon the magnitude of RDCs is quantitatively compared using three new models (elliptic, uni-dimensional, and equally populated two site jump) and three established models (static, isotropic motion in a cone and free diffusion about a fixed symmetry axis: Woessner's model) for motional averaging in the limit that the amplitude of motion βmax≤ 15-20°. The influence of the different motional models on the value of Robsdetermined from the distribution of RDCs is explored. The consequences of the different types of angular motion for the accurate determination of bond vector orientation, with respect to the alignment tensor, A, is investigated. The extent to which motion influences the magnitude of RDCs is compared to some non-dynamic factors affecting RDC size. © 2004 Elsevier Inc. All rights reserved.

Author-supplied keywords

  • Motional averaging
  • NMR
  • Residual dipolar coupling

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free