The retinoblastoma protein is phosphorylated during specific phases of the cell cycle

  • Buchkovich K
  • Duffy L
  • Harlow E
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p105-RB is the product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to act as an inhibitor of cellular proliferation, yet surprisingly it is present in actively dividing cells. To look for changes in p105-RB that may regulate its activity during the cell cycle, we generated synchronized cell populations and followed their progression through the cell cycle. p105-RB is synthesized throughout the cycle, but is phosphorylated in a phasespecific manner. In the G0 and G1 phases of the cell cycle, an unphosphorylated species of the protein is the only detectable form, whereas in the S and G2 M phases, multiple phosphorylated species of p105-RB are detected. © 1989.

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  • Karen Buchkovich

  • Linda A. Duffy

  • Ed Harlow

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