Reversed-Phase high-performance liquid chromatographic analysis of triacylglycerol autoxidation products with ultraviolet and evaporative light-scattering detectors

  • Viinanen E
  • Hopia A
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Abstract

The oxidation products of triacylgycerol standards (trilinolenin, trilinolein and triolein) and a natural mixture of rapeseed oil triacylglycerols were analyzed with a reversed-phase high-performance liquid chromatographic method. The oxidation products were detected with ultraviolet (UV) and evaporative light-scattering detectors (ELSD). The chromatographic profiles obtained with these two detectors were similar for all samples except triolein. ELSD is a mass detector and can detect the oxidation products of triolein and other compounds that do not have conjugated dienes in their structure. The two detectors can be used in series. The sensitivity of ELSD approached that of the UV detector used. ELSD seems to be a good universal detector type for monitoring autoxidation products of edible oils.

Author-supplied keywords

  • Autoxidation
  • ELSD
  • RP-HPLC
  • evaporative light-scattering detector
  • triacylglycerols

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Authors

  • Eija Viinanen

  • Anu Hopia

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