Rodent Immunohistochemistry: Pitfalls and Troubleshooting

  • Ward J
  • Rehg J
  • 65


    Mendeley users who have this article in their library.
  • 7


    Citations of this article.


Immunohistochemistry (IHC) is a common adjunct in pathology for morphologic diagnosis, research pathology, and studying the pathogenesis of the disease. Proper technique and interpretation of an immunohistochemistry assay is of utmost importance. A variety of problems, including the presence of artifacts (nonspecific background or other staining problems) and the differentiation between nonspecific and specific staining, commonly occur. It is essential that antibody quality and IHC technique be optimized. We review the histologic patterns of specific and nonspecific staining after using IHC techniques, as well as basic troubleshooting procedures, and provide some examples of nonspecific staining and other artifacts especially in formalin-fixed, paraffin-embedded tissues (FFPE) of mice.

Author-supplied keywords

  • IHC
  • antibodies
  • artifacts
  • background staining
  • immunohistochemistry
  • mice
  • mouse pathology

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • J. M. Ward

  • J. E. Rehg

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free