Role of Ascorbic Acid in Periodontal Ligament Cell Differentiation

  • Ishikawa S
  • Iwasaki K
  • Komaki M
 et al. 
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Abstract

Background: Periodontal ligament (PDL) cells form mineralized nodules in vitro. Ascorbic acid is known to be required in this process, although its effect on osteoblastic differentiation of PDL cells remains unclear. The purpose of this study was to determine the role of ascorbic acid on the early osteoblastic differentiation of PDL cells, with regard to alkaline phosphatase (ALP) activity, type I collagen production and integrin expression. Methods: Cultured PDL cells were stimulated at confluence with ascorbic acid in the presence or absence of type I collagen inhibitor and blocking antibodies to integrins. After stimulation, the cells and culture supernatants were examined for ALP activity, type I collagen production, and integrin expression. The ALP activity was measured using a colorimetric assay with p-nitrophenyl phosphate and ALP staining. Enzyme-linked immunosorbent assay (ELISA) was used to determine type I collagen production, and ELISA and flow cytometric analysis were employed for assessment ...

Author-supplied keywords

  • Alkaline phosphatase
  • ascorbic acid
  • cells, periodontal
  • collagen
  • integrins
  • osteoblasts
  • periodontal ligament

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Authors

  • Satoshi Ishikawa

  • Kengo Iwasaki

  • Motohiro Komaki

  • Isao Ishikawa

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