Conference proceedings

A role for matrix metalloproteinases and tumor host interaction in hepatocellular carcinomas

McKenna G, Chen Y, Smith R, Meneghetti A, Ong C, McMaster R, Scudamore C, Chung S ...see all

American Journal of Surgery, vol. 183, issue 5 (2002) pp. 588-594

  • 6

    Readers

    Mendeley users who have this article in their library.
  • 45

    Citations

    Citations of this article.
  • 1.5k

    Views

    ScienceDirect users who have downloaded this article.
Sign in to save reference

Abstract

Background: Hepatocellular carcinoma (HCC) occur in livers with injury-remodeling, accomplished by enzymes called matrix metalloproteinases (MMP). Metastasis involves basement membrane invasion also caused by MMP activity. Alterations in MMP expression and their endogenous inhibitor (TIMP) may factor in HCC metastasis. Methods: HCC specimens and lymph nodes (n = 7), and normal lymph tissue from organ donors (n = 8), were snap-frozen in liquid nitrogen and the mRNA precipitated. A series of reverse transcription-polymerase chain reactions (RT-PCR) were performed using MMP (MMP2, MMP7, MMP9) primers and TIMP (TIMP1, TIMP2) primers. These were semiquantitatively analyzed by comparing concentration with constitutive GADPH expression. Results: There is an increase in MMP2:TIMP2 mRNA expression ratio in the normal and tumor margin tissue compared to the tumor. There are increases in all MMP and TIMP mRNA expression (except TIMP1) and alterations in all of the MMP:TIMP expression ratios in the draining lymph node. Conclusions: Alterations exist in MMP2:TIMP2: expression at the margin, and all of the MMPs in the draining lymph nodes. This likely reflects a host-tumor interaction that regulates tumor metastasis. © 2002 Excerpta Medica, Inc. All rights reserved.

Author-supplied keywords

  • Hepatocellular carcinoma
  • MMP
  • Matrix metalloproteinase
  • Metastasis
  • TIMP
  • Tissue inhibitor of metalloproteinase

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text

Authors

  • Gregory J. McKenna

  • Yongliang Chen

  • R. Matt Smith

  • Adam Meneghetti

  • Christopher Ong

  • Robert McMaster

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free