A Salmonella protein causes macrophage cell death by inducing autophagy

  • Hernandez L
  • Pypaert M
  • Flavell R
 et al. 
  • 113


    Mendeley users who have this article in their library.
  • 156


    Citations of this article.


Salmonella enterica, the causative agent of food poisoning and typhoid fever, induces programmed cell death in macrophages, a process found to be dependent on a type III protein secretion system, and SipB, a protein with membrane fusion activity that is delivered into host cells by this system. When expressed in cultured cells, SipB caused the formation of and localized to unusual multimembrane structures. These structures resembled autophagosomes and contained both mitochondrial and endoplasmic reticulum markers. A mutant form of SipB devoid of membrane fusion activity localized to mitochondria, but did not induce the formation of membrane structures. Upon Salmonella infection of macrophages, SipB was found in mitochondria, which appeared swollen and devoid of christae. Salmonella-infected macrophages exhibited marked accumulation of autophagic vesicles. We propose that Salmonella, through the action of SipB, kills macrophages by disrupting mitochondria, thereby inducing autophagy and cell death.

Author-supplied keywords

  • Apoptosis
  • Bacterial pathogenesis
  • Host-pathogen interactionl
  • Membrane fusion
  • Type III protein secretion

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text


  • Lorraine D. Hernandez

  • Marc Pypaert

  • Richard A. Flavell

  • Jorge E. Galán

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free