Screening methods for synthetic activity of lipases

  • Sandoval G
  • Marty A
  • 71


    Mendeley users who have this article in their library.
  • 23


    Citations of this article.


In order to optimize synthetic biocatalytic transformations catalyzed by lipases, a large number of enzyme preparations must be tested. Moreover, to design an enzyme for an industrial application it is generally necessary to realize enzyme evolution to improve enzyme performances, for instance thermostability, activity or selectivity. The crucial step is then the screening of the optimal catalyst. Because hydrolytic and synthetic activities are not always correlated, specific screening methods for synthesis activity of lipases are needed. In this work, we report three sensitive and specific screening methods to measure synthetic activity of lipases in vivo on an agar plate and in vitro, directly from culture broth. © 2006 Elsevier Inc. All rights reserved.

Author-supplied keywords

  • Lipase
  • Screening
  • Synthetic activity

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free