Important biological molecules such as collagen and myosin are strong second harmonic generators. The polarization dependence of second-harmonic generation (SHG) is a property of the second order susceptibility elements (chi tensor) of the molecules that produce the signal. In this work, we used polarization SHG microscopy to image and map pixel scale polarization anisotropy information of collagen-muscle junction of chicken wing and the dermis of human skin. We show that the variation in the chi tensor components can be displayed as images to show the contrast between different molecular sources of SHG.
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