Selective breast cancer cell capture, culture, and immunocytochemical analysis using self-assembled magnetic bead patterns in a microfluidic chip.

  • Sivagnanam V
  • Song B
  • Vandevyver C
 et al. 
  • 16

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Abstract

Separation and subsequent culturing of MCF-7 breast cancer cells on self-assembled protein-coated magnetic beads in a microfluidic chip is demonstrated. The beads were patterned in situ inside a sealed microfluidic channel using magnetic-field-assisted electrostatic self-assembly. Hereafter, they were grafted by exposure to a solution of 5D10 monoclonal antibodies (mAb) and fibronectin (FN), with the first being used for immunospecific cell capture and the latter being used for cell adhesion and growth. A solution of target MCF-7 cells mixed with Jurkat cells was brought inside the microchannel, leading to specific MCF-7 cell capture; the latter were then cultured and evidenced by cell immuno-luminescence.

Author-supplied keywords

  • Biological Markers
  • Biological Markers: metabolism
  • Breast Neoplasms
  • Breast Neoplasms: genetics
  • Breast Neoplasms: metabolism
  • Breast Neoplasms: pathology
  • Cell Adhesion
  • Cell Culture Techniques
  • Cell Culture Techniques: instrumentation
  • Cell Separation
  • Cell Separation: instrumentation
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immunohistochemistry
  • Jurkat Cells
  • Luminescent Measurements
  • Magnetics
  • Microfluidic Analytical Techniques
  • Microspheres

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Authors

  • Venkataragavalu Sivagnanam

  • Bo Song

  • Caroline Vandevyver

  • Jean-Claude G Bünzli

  • Martin a M Gijs

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