From SELEX to cell: Dual selections for synthetic riboswitches

  • Sinha J
  • Topp S
  • Gallivan J
  • 45


    Mendeley users who have this article in their library.
  • 7


    Citations of this article.


Synthetic riboswitches have emerged as useful tools for controlling gene expression to reprogram cellular behavior. However, advancing beyond proof-of-principle experiments requires the ability to quickly generate new synthetic riboswitches from RNA libraries. In this chapter, we provide a step-by-step overview of the process of obtaining synthetic riboswitches for use in Escherichia coli, starting from a randomized RNA library. © 2011 Elsevier Inc. All rights reserved.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Joy Sinha

  • Shana Topp

  • Justin P. Gallivan

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free