From SELEX to cell: Dual selections for synthetic riboswitches

  • Sinha J
  • Topp S
  • Gallivan J
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Abstract

Synthetic riboswitches have emerged as useful tools for controlling gene expression to reprogram cellular behavior. However, advancing beyond proof-of-principle experiments requires the ability to quickly generate new synthetic riboswitches from RNA libraries. In this chapter, we provide a step-by-step overview of the process of obtaining synthetic riboswitches for use in Escherichia coli, starting from a randomized RNA library. © 2011 Elsevier Inc. All rights reserved.

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Authors

  • Joy Sinha

  • Shana Topp

  • Justin P. Gallivan

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