From SELEX to cell: Dual selections for synthetic riboswitches

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Abstract

Synthetic riboswitches have emerged as useful tools for controlling gene expression to reprogram cellular behavior. However, advancing beyond proof-of-principle experiments requires the ability to quickly generate new synthetic riboswitches from RNA libraries. In this chapter, we provide a step-by-step overview of the process of obtaining synthetic riboswitches for use in Escherichia coli, starting from a randomized RNA library. © 2011 Elsevier Inc. All rights reserved.

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Sinha, J., Topp, S., & Gallivan, J. P. (2011). From SELEX to cell: Dual selections for synthetic riboswitches. Methods in Enzymology, 497, 207–220. https://doi.org/10.1016/B978-0-12-385075-100009-3

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