Sequence variation in the cadherin gene of Ostrinia nubilalis: A tool for field monitoring

  • Coates B
  • Sumerford D
  • Hellmich R
 et al. 
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Abstract

Toxin-binding proteins of insect midgut epithelial cells are associated with insect resistance to Bacillus thuringiensis (Bt) Cry toxins. A 5378 nt cDNA encoding a 1717 amino acid putative midgut cadherin-like glycoprotein and candidate Cry1Ab toxin-binding protein was characterized from Ostrinia nubilalis. Intraspecific alignment of partial O. nubilalis cadherin gene sequences identified variance within proposed Cry1A toxin binding region 2 (TBR2),1328IPLQTSILVVT[I/V] N1340, and flanking Cry1A toxin binding region 1 (TBR1),861DIEIEIIDTNN871. DNA sequence and PCR-RFLP detected single nucleotide polymorphism between cadherin alleles, and pedigree analysis demonstrated Mendelian inheritance. A population sample from Mead, Nebraska showed allelic polymorphism. These assays may be useful for linkage mapping and field surveillance of wild populations and of O. nubilalis.

Author-supplied keywords

  • Bacillus thuringiensis receptor
  • Cadherin
  • Midgut cDNA

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Authors

  • Brad S. Coates

  • Douglas V. Sumerford

  • Richard L. Hellmich

  • Leslie C. Lewis

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