A serum shock induces circadian gene expression in mammalian tissue culture cells

  • Balsalobre A
  • Damiola F
  • Schibler U
  • 2


    Mendeley users who have this article in their library.
  • N/A


    Citations of this article.


The treatment of cultured rat-1 fibroblasts or H35 hepatoma cells with high concentrations of serum induces the circadian expression of various genes whose transcription also oscillates in living animals. Oscillating genes include rper1 and rper2 (rat homologs of the Drosophila clock gene period), and the genes encoding the transcription factors Rev-Erb alpha, DBP, and TEF. In rat-1 fibroblasts, up to three consecutive daily oscillations with an average period length of 22.5 hr could be recorded. The temporal sequence of the various mRNA accumulation cycles is the same in cultured cells and in vivo. The serum shock of rat-1 fibroblasts also results in a transient stimulation of c-fos and rper expression and thus mimics light-induced immediate-early gene expression in the suprachiasmatic nucleus

Author-supplied keywords

  • Animals
  • Biological Clocks
  • Carcinoma,Hepatocellular
  • Cell Cycle
  • Cell Cycle Proteins
  • Cell Line
  • Circadian Rhythm
  • Cycloheximide
  • Drosophila
  • Fibroblasts
  • Gene Expression
  • Gene Expression Regulation
  • Genes
  • Genes,Immediate-Early
  • Genes,fos
  • Inhibitor
  • Liver
  • Nuclear Proteins
  • Period Circadian Proteins
  • Protein Synthesis Inhibitors
  • Proteins
  • RNA,Messenger
  • RNA,Neoplasm
  • Rats
  • Rna
  • Suprachiasmatic Nucleus
  • Switzerland
  • Transcription Factors
  • Tumor Cells,Cultured
  • biosynthesis
  • blood
  • drug effects
  • genetics
  • metabolism
  • pharmacology
  • physiology

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

  • PMID: 9635423


  • A Balsalobre

  • F Damiola

  • U Schibler

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free