Short-term incubation of bovine placentome sections as a tool to study xenobiotic mechanism of action

  • Wojciechowska A
  • Młynarczuk J
  • Kotwica J
  • 2


    Mendeley users who have this article in their library.
  • 2


    Citations of this article.


Studies on the effects of various factors, including xenobiotics, on the maternal-fetal connections in the placenta are restricted by the lack of a simple and inexpensive research model. We used placentomes collected at a slaughterhouse to in vitro study the bovine sections contained integral maternal-fetal connections. The placentomes from cows (n=4/experiment, 120-150 days post coitum) were cut using a razor blade into 60-80mg sections and incubated in either DMEM/Ham's F-12 or M-199 supplemented with FCS (2%, 5% or 10%), amniotic fluid (AF or inactive AF, 10% or 20%) or both. The sections (n=4/supplement) were incubated for 24 or 48h in a water bath at 37.5°C in an atmosphere of 5% CO2and 95% O2. The structure and secretory activity of placentome sections were maintained when incubated in DMEM/Ham's F-12 with 2% FCS and 10% AF. M-199 was less acidified than DMEM/Ham's F-12 during incubation, and thus, this medium was better able to maintain the integrity of the placenta and the secretion of estradiol, progesterone and oxytocin for 48h. Moreover, we detected a decrease in the expression of placenta-specific 1 (PLAC1) mRNA (an indicator of trophoblast proliferation) and an increase in the levels of keratin 8 (KRT8; a marker of normal placental barrier function) and hypoxia induced factor 1α (HIF1α; a marker of hypoxia) mRNA. These results indicate the presence of adaptation and repair mechanisms and confirm the biological activity of the placentome sections. We propose the use of placentome sections as an in vitro model to study maternal-fetal connections in cows.

Author-supplied keywords

  • Cow
  • Fetal-maternal connections
  • In vitro incubations
  • Placenta
  • Placentomes

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text


  • Anna Wojciechowska

  • Jarosław Młynarczuk

  • Jan Kotwica

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free