Significance of CD90+ cancer stem cells in human liver cancer

  • Yang Z
  • Ho D
  • Ng M
 et al. 
  • 25

    Readers

    Mendeley users who have this article in their library.
  • N/A

    Citations

    Citations of this article.

Abstract

This study characterized cancer stem cells (CSCs) in hepatocellular carcinoma (HCC) cell lines, tumor specimens, and blood samples. The CD90+ cells, but not the CD90(-) cells, from HCC cell lines displayed tumorigenic capacity. All the tumor specimens and 91.6% of blood samples from liver cancer patients bore the CD45(-)CD90+ population, which could generate tumor nodules in immunodeficient mice. The CD90+CD44+ cells demonstrated a more aggressive phenotype than the CD90+CD44(-) counterpart and formed metastatic lesions in the lung of immunodeficient mice. CD44 blockade prevented the formation of local and metastatic tumor nodules by the CD90+ cells. Differential gene expression profiles were identified in the CD45(-)CD90+ and CD45(-)CD90(-) cells isolated from tissue and blood samples from liver cancer patients and controls.

Author-supplied keywords

  • Animals
  • Antigens, CD44/metabolism
  • Antigens, CD45/metabolism
  • Antigens, Thy-1/*metabolism
  • Apoptosis
  • Carcinoma, Hepatocellular/genetics/pathology
  • Cell Line, Tumor
  • Cell Separation
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Liver Neoplasms/genetics/*pathology
  • Male
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Neoplastic Stem Cells/*metabolism/*pathology
  • Reproducibility of Results
  • Reverse Transcriptase Polymerase Chain Reaction

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Authors

  • Z F Yang

  • D W Ho

  • M N Ng

  • C K Lau

  • W C Yu

  • P Ngai

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free