A simple and efficient method for purification of intact white spot syndrome virus (WSSV) viral particles

  • Xie X
  • Li H
  • Xu L
 et al. 
  • 4

    Readers

    Mendeley users who have this article in their library.
  • N/A

    Citations

    Citations of this article.

Abstract

A new simple and efficient method for isolation of intact WSSV viral particles from infected crayfish tissues with high yield was developed. Abundant viral particles could be obtained with only a few steps of conventional differential centrifugations, while no density gradient centrifugation or ultracentrifugation was required. The concentrated virus preparations were further studied by transmission electron microscopy and polyacrylamide gel electrophoresis. Using negative-staining TEM, we found that purified viral particles were coated with integral envelope. At least 23 major structural proteins from purified WSSV virions could be observed by SDS-PAGE. By this method, about 1012 viral particles could be recovered from 10 g of infected crayfish tissues. Moreover, purified virus does not lose its biological activity. Using purified virus, the minimal amount of WSSV that could initiate a successful virus proliferation in crayfish was determined. © 2004 Elsevier B.V. All rights reserved.

Author-supplied keywords

  • Structural proteins
  • Virus infectivity
  • Virus purification
  • Virus quantification
  • White spot syndrome virus

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

There are no full text links

Authors

  • X Xie

  • H Li

  • L Xu

  • F Yang

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free