A simple procedure for the isolation of high quality rna from ripening banana fruit

  • Asif M
  • Dhawan P
  • Nath P
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Isolation of high quality RNA from ripening banana fruit tissue is difficult due to high levels of polysaccharides and other substances that interfere when using conven-tional procedures for RNA isolation. These substances not only decrease the yield but the quality of RNA is almost unusable. We describe here a simple RNA procedure that effec-tively removes these contaminating substances without affecting the yield. Following this procedure, we routinely obtained 80-150 ~tg of total RNA per g fresh tissue. The RNA is of good quality and suitable for northern analysis, RT-PCR and cDNA library construction.

Author-supplied keywords

  • Banana
  • Fruit tissue
  • Musa acuminata
  • Polysaccharides
  • Rna extraction

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  • Mehar H. Asif

  • Puneet Dhawan

  • Pravendra Nath

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