Simultaneous changes in cell quotas of microcystin, chlorophyll a, protein and carbohydrate during different growth phases of a batch culture experiment with Microcystis aeruginosa

  • Lyck S
  • 55

    Readers

    Mendeley users who have this article in their library.
  • 50

    Citations

    Citations of this article.

Abstract

The cell quotas of microcystin (Qmcyst), protein (Qprot), chlorophyll a (Qchloro) and carbohydrate (Qcarbo), as well as the net production rates of these parameters, were determined during the exponential and stationary phases in nine batch cultures of Microcystis aeruginosa (CYA 228) at light regimes from 33 to 53 {micro}mol photons m-2 s-1. The following results were obtained. (i) A parallel pattern was found in the changes of Qmcyst, Qprot, Qchloro and Qcarbo during the entire growth cycle and significant correlations were recorded between Qmcyst and Qprot, Qchloro and Qcarbo. (ii) The net microcystin production rate ({micro}mcyst) was positively correlated with the specific cell division rate ({micro}c), the chlorophyll production rate ({micro}chloro) and the protein production rate. (iii) A significant inverse linear relationship was found between {micro}c and Qmcyst, i.e. cultures with a positive {micro}c had a Qmcyst between 110 and 400 fg microcystin cell-1, while declining cultures had Qmcyst values >400 fg microcystin cell-1. Maximum variation in Qmcyst within cultures was 3.5-fold. Collectively, the results show that cells produced microcystin at rates approximating those needed to replace losses to daughter cells during division and that microcystin was produced in a similar way to protein and chlorophyll, indicating a constitutive microcystin production.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Susanne Lyck

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free