Simultaneous determination of tyrosine, tryptophan and 5-hydroxytryptamine in serum of MDD patients by high performance liquid chromatography with fluorescence detection

  • Sa M
  • Ying L
  • Tang A
 et al. 
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Abstract

Background: Tyrosine (Tyr), Tryptophan (Trp) and 5-hydroxytryptamine (5-HT) are important amino acids in vivo and have been hypothesized to be involved in many mental disorders. We developed a rapid and sensitive HPLC method for simultaneous measurement of serum Tyr, Trp and 5-HT and explored the clinical significances of Tyr, Trp and 5-HT and the 5-HT/Trp ratio for patients with major depressive disorder (MDD) disease. Methods: Serum samples were deproteinized by 5% perchloric acid and separated on an Atlantis C18 column (4.6×150mm, 5μm) with the mobile phase consisting of 0.1mol/l KH2PO4and methanol (85:15, V/V).The eluates were monitored by the fluorescence detection with programmed wavelength. Results: Analysis was achieved in

Author-supplied keywords

  • 5-hydroxytryptamine
  • Fluorescence detection
  • High performance liquid chromatography
  • Major depressive disorder
  • Tryptophan
  • Tyrosine

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