Simultaneous qualitative and quantitative analyses of the major constituents in the rhizome of Ligusticum Chuanxiong using HPLC-DAD-MS

  • Yi T
  • Leung K
  • Lu G
 et al. 
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Abstract

An HPLC-DAD-MS method was developed for the qual. and quant. anal. of the major constituents in Chuanxiong (the dried rhizome of Ligusticum chuanxiong Hort). Twenty compds. including phenolic constituents, alkylphthalides and phthalide dimers were identified using online ESI-MS and comparisons with literature data and std. compds., and 6 of them were quantified by HPLC-DAD simultaneously. A comprehensive validation of the method including sensitivity, linearity, repeatability and recovery was conducted. The linear regressions were acquired with R2>0.99 and limit of detection (LOD, S/N = 3) values were between 1.5 and 2.5 ng. The repeatability was evaluated by intra- and inter-day assays, and relative std. deviation (RSD) values were reported within 1.87%. The recovery studies for the quantified compds. were obsd. in the range of 96.36-102.37% with RSD values less than 2.63%. These phenolic constituents and alkylphthalides, the major constituents in Chuanxiong, are generally regarded as the index for the quality assessment of this herb. The overall procedure is accurate and reproducible, which is considered suitable for the qual. and quant. analyses of a large no. of Chuanxiong samples. [on SciFinder(R)]

Author-supplied keywords

  • alkylphthalide
  • chinese
  • chuang-xiong in
  • chuanxiong
  • hplc-dad-ms
  • ligusticum chuanxiong
  • ligusticum chuanxiong hort
  • phenolic constituent
  • quality assessment
  • the dried rhizome of
  • tousenkyu in japanese and

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Authors

  • Tao Yi

  • Kelvin Sze-Yin Leung

  • Guang-Hua Lu

  • Kelvin Chan

  • Hao. Zhang

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