A small aptamer with strong and specific recognition of the triphosphate of ATP

  • Sazani P
  • Larralde R
  • Szostak J
  • 109


    Mendeley users who have this article in their library.
  • 103


    Citations of this article.


We report the in vitro selection of an RNA-based ATP aptamer with the ability to discriminate between adenosine ligands based on their 5' phosphorylation state. Previous selection of ATP aptamers yielded molecules that do not significantly discriminate between ligands at the 5' position. By applying a selective pressure that demands recognition of the 5' triphosphate, we obtained an aptamer that binds to ATP with a Kd of approximately 5 muM, and to AMP with a Kd of approximately 5.5 mM, a difference of 1100-fold. This aptamer demonstrates the ability of small RNAs to interact with negatively charged moieties.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Peter L. Sazani

  • Rosa Larralde

  • Jack W. Szostak

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free