Small RNA RyhB as a potential tool used for metabolic engineering in Escherichia coli

  • Kang Z
  • Wang X
  • Li Y
 et al. 
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Abstract

Small RNA (RyhB) was overexpressed artificially using an arabinose-inducible system in Escherichia coli and resulted in more succinate (7-fold) accumulation, which suggested that RyhB had a strong effect on sdhCDAB genes. Acetate was also increased indicating that RyhB had a comprehensive influence on glucose central metabolism. RyhB might therefore be useful for metabolic engineering of E. coli.

Author-supplied keywords

  • *Gene Expression
  • *Gene Expression Regulation, Bacterial
  • Acetates/metabolism
  • Escherichia coli/*genetics
  • Glucose/metabolism
  • Metabolic Engineering/*methods
  • RNA, Small Untranslated/*genetics/metabolism
  • Succinic Acid/metabolism

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Authors

  • Z Kang

  • X Wang

  • Y Li

  • Q Wang

  • Q Qi

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