S-methylthioacetimidate is a new reagent for the amidination of proteins at low pH

  • Thumm M
  • Hoenes J
  • Pfleiderer G
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Abstract

S-Methylthioacetimidate is a novel amidation reagent which can be used even as low as pH 5 without any side of reaction or crosslinking of the protein. At pH 6, a complete modification of all accessible lysines of bovine serum albumin, pig heart lactate dehydrogenase and glucose dehydrogenase from Bacillus megaterium could be achieved by a single addition of reagent. The protection of the essential lysine of glucose dehydrogenase (B. megaterium) was significantly improved when using thioacetimidate at pH 6.0 instead of the O-acetimidate at pH 8.5. The residual activity was 70% with the thiomidate in contrast to 10% with the normally used )-acetimidate. © 1987.

Author-supplied keywords

  • Amidination
  • Protein modification
  • S-Methylthioacetimidate
  • low pH

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Authors

  • Michael Thumm

  • Joachim Hoenes

  • Gerhard Pfleiderer

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