Sonic activation of molecularly-targeted nanoparticles accelerates transmembrane lipid delivery to cancer cells through contact-mediated mechanisms: Implications for enhanced local drug delivery

  • Crowder K
  • Hughes M
  • Marsh J
 et al. 
  • 40


    Mendeley users who have this article in their library.
  • 62


    Citations of this article.


Liquid perfluorocarbon nanoparticles serve as sensitive and specific targeted contrast and drug delivery vehicles by binding to specific cell surface markers. We hypothesized that application of acoustic energy at diagnostic power levels could promote nanoparticle-associated drug delivery by stimulating increased interaction between the nanoparticle's lipid layer and the targeted cell's plasma membrane. Ultrasound (mechanical index = 1.9) applied with a conventional ultrasound imaging system to nanoparticles targeted to αvβ3-integrins on C32 melanoma cancer cells in vitro produced no untoward effects. Within 5 min, lipid delivery from nanoparticles into cell cytoplasm was dramatically augmented. We also demonstrate the operation of a potential physical mechanism for this effect, the acoustic radiation force on the nanoparticles, which may contribute to the enhanced lipid delivery. Accordingly, we propose that local delivery of lipophilic substances (e.g., drugs) from targeted nanoparticles directly into cell cytoplasm can be augmented rapidly and safely with conventional ultrasound imaging devices through nondestructive mechanisms. © 2005 World Federation for Ultrasound in Medicine & Biology.

Author-supplied keywords

  • Acoustic radiation forces
  • Contrast agent
  • Lipid exchange
  • Membrane fusion
  • Nanoparticle
  • Targeted drug delivery
  • Ultrasound

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Kathryn C. Crowder

  • Michael S. Hughes

  • Jon N. Marsh

  • Alejandro M. Barbieri

  • Ralph W. Fuhrhop

  • Gregory M. Lanza

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free