Spatiotemporal analysis of cell response to a rigidity gradient: a quantitative study using multiple optical tweezers.

  • Allioux-Gu�rin M
  • Icard-Arcizet D
  • Durieux C
 et al. 
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Abstract

We investigate the dynamic response of single cells to weak and local
rigidities, applied at controlled adhesion sites. Using multiple
latex beads functionalized with fibronectin, and each trapped in
its own optical trap, we study the reaction in real time of single
3T3 fibroblast cells to asymmetrical tensions in the tens of pN x
microm(-1) range. We show that the cell feels a rigidity gradient
even at this low range of tension, and over time develops an adapted
change in the force exerted on each adhesion site. The rate at which
force increases is proportional to trap stiffness. Actomyosin recruitment
is regulated in space and time along the rigidity gradient, resulting
in a linear relationship between the amount of recruited actin and
the force developed independently in trap stiffness. This time-regulated
actomyosin behavior sustains a constant and rigidity-independent
velocity of beads inside the traps. Our results show that the strengthening
of extracellular matrix-cytoskeleton linkages along a rigidity gradient
is regulated by controlling adhesion area and actomyosin recruitment,
to maintain a constant deformation of the extracellular matrix.

Author-supplied keywords

  • 3T3 Cells; Actins
  • Mechanical; Time Factors
  • metabolism; Actomyosin
  • metabolism; Animals; Cell Adhesion
  • metabolism; Linear Models; Mice; Motion; Myosin T
  • metabolism; Optical Tweezers; Stress
  • physiology; Cell Physiological Phenomena
  • physiology; Cytoskeleton
  • physiology; Elasticity; Extracellular Matrix
  • physiology; Fibroblasts
  • physiology; Fibronectins

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Authors

  • Myriam Allioux-Gu�rin

  • Delphine Icard-Arcizet

  • Christiane Durieux

  • Sylvie H�non

  • Fran�ois Gallet

  • Jean-Claude Mevel

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