Species-specific detection of predation by coleoptera on the milacid slug Tandonia budapestensis (Mollusca: Pulmonata)

  • Symondson W
  • Erickson M
  • Liddell J
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Abstract

Tandonia budapestensis is one of the most damaging species of slug in the UK , and can cause major losses in potatoes and cereals . No previous attempts appear to have been made to investigate the potential of natural enemies to control this slug . Monoclonal antibodies can be used to analyze the crop contents of predators collected from the field , in order to detect and quantify the remains of major pest species and identify important natural enemies . A species - specific antibody was therefore raised that could distinguish between T. budapestensis and all other molluscs tested . The antibody could detect as little as 5.57 ng of prey protein in an enzyme - linked immunosorbent assay . It could clearly identify the remains of T. budapestensis among the crop contents of the carabid Pterostichus melanarius for more than 26 h at 16 o C , while the half - life of the antigen within the predator was 12.8 h . Such intervals make it a highly suitable antibody probe for quantifying predation during the previous...

Author-supplied keywords

  • Crop ecology
  • Detection limit
  • ELISA
  • Milax
  • Monoclonal antibody
  • Predation
  • Pterostichus melanarius
  • Tandonia budapestensis

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Authors

  • W. O.C. Symondson

  • M. L. Erickson

  • J. E. Liddell

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