Specific amplification of 18S fungal ribosomal genes from vesicular- arbuscular endomycorrhizal fungi colonizing roots

  • Simon L
  • Lalonde M
  • Bruns T
  • 153

    Readers

    Mendeley users who have this article in their library.
  • 433

    Citations

    Citations of this article.

Abstract

The first DNA sequences obtained from arbuscular endomycorrhizal fungi are reported. They were obtained by directly sequencing overlapping amplified fragments of the nuclear genes coding for the small subunit rRNA. These sequences were used to develop a polymerase chain reaction primer (VANS1) that enables the specific amplification of a portion of the vesicular-arbuscular endomycorrhizal fungus small subunit rRNA directly from a mixture of plant and fungal tissues. The specificity of this primer for arbuscular endomycorrhizal fungi was demonstrated by testing it on a number of organisms and by sequencing the fragment amplified from colonized leek (Allium porum) roots. This approach, coupled with other molecular techniques, will facilitate rapid detection, identification, and possibly quantitation of arbuscular endomycorrhizal fungi.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

  • ISBN: 0099-2240
  • SGR: 0026513188
  • PMID: 1339260
  • PUI: 22039228
  • SCOPUS: 2-s2.0-0026513188
  • ISSN: 00992240

Authors

  • L. Simon

  • M. Lalonde

  • T. D. Bruns

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free