Specific PCR primers to identify arbuscular mycorrhizal fungi within colonized roots

  • Redecker D
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A set of PCR primers targeted at five major phylogenetic subgroups of arbuscular mycorrhizal fungi (Glomales) was designed to facilitate specific amplifica- tion of internal transcribed spacers and 18 S rRNA gene fragments from colonized roots in the absence of spores. The subgroups include the recently discovered deeply divergent lineages of Glomales, which could not be detected by previously reported PCR primers, and the former genus Sclerocystis. Restriction fragment length polymorphism patterns presented allow identifi- cation of presently known members of these groups. The resulting PCR products can be used to identify the fungal symbionts at the genus or species level by re- striction digests or DNA sequencing. A novel DNA ex- traction method allows visual control of the analyzed roots by staining procedures after analysis by PCR.

Author-supplied keywords

  • DNA extraction
  • Glomales
  • Internal transcribed spacers
  • Molecular identification
  • RFLP patterns

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  • D. Redecker

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