Spectral imaging fluorescence microscopy

  • Haraguchi T
  • Shimi T
  • Koujin T
 et al. 
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Abstract

... at an exitation wavelength of 484 nm and at 527 nm are plotted as a function of time; the acceptor signal (at 527 nm) increased and the donor signal (at 484 nm) decreased following the induction, indicating that FRET occurred. In spectral imaging , spectral changes can be ...

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