Spectrum of Lactobacillus species present in healthy vagina of Indian women

  • Garg K
  • Ganguli I
  • Das R
 et al. 
  • 31

    Readers

    Mendeley users who have this article in their library.
  • 25

    Citations

    Citations of this article.

Abstract

BACKGROUND & OBJECTIVE: Lactobacilli are depleted in vagina of women suffering from recurring episodes of bacterial vaginosis with vaginal pH >or=5. With the objective of making available probiotic lactobacilli for replenishment in such women, a study was undertaken to isolate and characterize the Lactobacilli present in women with eco-healthy vagina in Delhi. No information is so far available on the species of Lactobacilli resident in vagina of women in India. METHODS: Vaginal swabs were taken from 80 women with informed consent after ethical approval and grown in MRS broth. Gram-positive, catalase-negative bacilli generating about 200 bp amplicon by PCR with Lactobacillus genus specific primers were further characterized by employing species specific primers followed by sequencing of 16S rDNA. Isolates of the same species were differentiated by random amplified polymorphic DNA (RAPD) profiles. RESULTS: The predominant species isolated were L. reuteri present in 26 (32.5%) women, L. fermentum in 20 (25%), and L. salivarius in 13 (16.25%) women. Sequencing of 16S rDNA of 20 isolates showed that except for two isolates of L. plantarum, sequences of the remaining agreed well with PCR identification. None of the isolates had similar RAPD profile. INTERPRETATION & CONCLUSION: Our findings showed lactobacilli species present in healthy vagina of women in India differ from those reported from other countries. This information would be useful to development of probiotic tablets seeking to replenish the missing lactobacilli for reproductive health of women in India.

Author-supplied keywords

  • 16S rDNA sequencing
  • Bacterial vaginosis
  • Lactobacillus
  • PCR
  • RAPD
  • Variable strains

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

  • ISSN: 09715916
  • PMID: 19692744
  • PUI: 355344219
  • SCOPUS: 2-s2.0-70349555972
  • SGR: 70349555972

Authors

  • Kavita Bansal Garg

  • Indrani Ganguli

  • Ram Das

  • G. P. Talwar

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free