Here we report a metabolomics discovery study conducted on blood serum samples of patients in different stages of chronic kidney disease (CKD). Metabolites were monitored on a quality controlled holistic platform combining reversed-phase liquid chromatography coupled to high-resolution quadrupole time-of-flight mass spectrometry in both negative and positive ionization mode and gas chromatography coupled to quadrupole mass spectrometry. A substantial portion of the serum metabolome was thereby covered. Eighty-five metabolites were shown to evolve with CKD progression of which 43 metabolites were a confirmation of earlier reported uremic retention solutes and/or uremic toxins. Thirty-one unique metabolites were revealed which were increasing significantly throughout CKD progression, by a factor surpassing the level observed for creatinine, the currently used biomarker for kidney function. Additionally, 11 unique metabolites showed a decreasing trend. © 2013 Springer Science+Business Media New York.
CITATION STYLE
Boelaert, J., t’Kindt, R., Schepers, E., Jorge, L., Glorieux, G., Neirynck, N., … Sandra, K. (2014). State-of-the-art non-targeted metabolomics in the study of chronic kidney disease. Metabolomics, 10(3), 425–442. https://doi.org/10.1007/s11306-013-0592-z
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