STD sensor based on nucleic acid functionalized nanostructured polyaniline

  • Singh R
  • Prasad R
  • Sumana G
 et al. 
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Abstract

STD (sexually transmitted disease, Gonorrhoea) sensor based on nucleic acid probe (from Opa, a multi-copy gene of Neisseria gonorrhoeae) functionalized nanostructured-polyaniline coated onto indium-tin-oxide-coated glass plate has been fabricated using avidin-biotin as cross-linking agent. This DNA functionalized electrode can specifically detect upto 0.5 × 10-15M of complementary target within 60 s of hybridization time at 25 °C by differential pulse voltammetry (DPV) using methylene blue as electro-active DNA hybridization indicator. This highly sensitive and specific nucleic acid functionalized nanostructured-polyaniline electrode can distinguish presence of N. gonorrhoeae from Neisseria meningitidis and Escherichia coli culture and spiked samples from the urethral swabs of the patients. © 2008 Elsevier B.V. All rights reserved.

Author-supplied keywords

  • DNA biosensor
  • Electrochemical characterization
  • Methylene blue
  • Neisseria gonorrhoeae
  • Polyaniline
  • Sexually transmitted disease

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Authors

  • Renu Singh

  • Rachna Prasad

  • G. Sumana

  • Kavita Arora

  • Seema Sood

  • R. K. Gupta

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