STD (sexually transmitted disease, Gonorrhoea) sensor based on nucleic acid probe (from Opa, a multi-copy gene of Neisseria gonorrhoeae) functionalized nanostructured-polyaniline coated onto indium-tin-oxide-coated glass plate has been fabricated using avidin-biotin as cross-linking agent. This DNA functionalized electrode can specifically detect upto 0.5 × 10-15 M of complementary target within 60 s of hybridization time at 25 °C by differential pulse voltammetry (DPV) using methylene blue as electro-active DNA hybridization indicator. This highly sensitive and specific nucleic acid functionalized nanostructured-polyaniline electrode can distinguish presence of N. gonorrhoeae from Neisseria meningitidis and Escherichia coli culture and spiked samples from the urethral swabs of the patients. © 2008 Elsevier B.V. All rights reserved.
CITATION STYLE
Singh, R., Prasad, R., Sumana, G., Arora, K., Sood, S., Gupta, R. K., & Malhotra, B. D. (2009). STD sensor based on nucleic acid functionalized nanostructured polyaniline. Biosensors and Bioelectronics, 24(7), 2232–2238. https://doi.org/10.1016/j.bios.2008.11.030
Mendeley helps you to discover research relevant for your work.