The structural determinants of checkpoint activation.

  • MacDougall C
  • Byun T
  • Van C
 et al. 
  • 57


    Mendeley users who have this article in their library.
  • N/A


    Citations of this article.


Here, we demonstrate that primed, single-stranded DNA (ssDNA) is sufficient for activation of the ATR-dependent checkpoint pathway in Xenopus egg extracts. Using this structure, we define the contribution of the 5'- and 3'-primer ends to Chk1 activation when replication is blocked and ongoing. In addition, we show that although ssDNA is not sufficient for checkpoint activation, the amount of ssDNA adjacent to the primer influences the level of Chk1 phosphorylation. These observations define the minimal DNA requirements for checkpoint activation and suggest that primed ssDNA represents a common checkpoint activating-structure formed following many types of damage.

Author-supplied keywords

  • Animals
  • Ataxia Telangiectasia Mutated Proteins
  • Cell Cycle
  • Cell Cycle Proteins
  • Cell Cycle Proteins: metabolism
  • DNA Damage
  • DNA Replication
  • DNA, Single-Stranded
  • DNA, Single-Stranded: metabolism
  • Ovum
  • Ovum: chemistry
  • Phosphorylation
  • Protein Kinases
  • Protein Kinases: metabolism
  • Protein-Serine-Threonine Kinases
  • Protein-Serine-Threonine Kinases: metabolism
  • S Phase
  • Xenopus Proteins
  • Xenopus Proteins: metabolism
  • Xenopus laevis

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Christina A MacDougall

  • Tony S Byun

  • Christopher Van

  • Muh-ching Yee

  • Karlene A Cimprich

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free