Subzero nonfreezing cryopresevation of rat hearts using antifreeze protein I and antifreeze protein III

  • Amir G
  • Horowitz L
  • Rubinsky B
 et al. 
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The purpose of the present study was to evaluate whether AFPs protect the heart from freezing and improve survival and viability in subzero cryopreservation. Hearts were subject to 5 preservation protocols; University of Wisconsin solution (UW) at 4°C, UW at -1.3°C without nucleation, UW at -1.3°C with nucleation, UW AFP I (15mg/cm3) at -1.3°C with nucleation, and in UW AFP III (15mg/cm3) at -1.3°C with nucleation. Hearts were preserved for 24, 28, and 32h, rewarmed and connected to the working isolated perfusion system. Data [heart rate (HR), coronary flow (CF), and developed pressure (dP)] was collected 30 and 60min after reperfusion. Hearts preserved at -1.3°C without AFPs froze, while hearts preserved with AFP did not freeze when nucleation was initiated and survived. Survival and dP of hearts preserved for 24h at -1.3°C using AFP III was better than those preserved at 4°C, (dP; 1.4 vs. 0.8, p

Author-supplied keywords

  • Antifreeze proteins
  • Cryopreservation
  • Heart
  • Preservation
  • Subzero

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