Subzero nonfreezing cryopresevation of rat hearts using antifreeze protein I and antifreeze protein III

  • Amir G
  • Horowitz L
  • Rubinsky B
 et al. 
  • 19

    Readers

    Mendeley users who have this article in their library.
  • 25

    Citations

    Citations of this article.

Abstract

The purpose of the present study was to evaluate whether AFPs protect the heart from freezing and improve survival and viability in subzero cryopreservation. Hearts were subject to 5 preservation protocols; University of Wisconsin solution (UW) at 4°C, UW at -1.3°C without nucleation, UW at -1.3°C with nucleation, UW AFP I (15mg/cm3) at -1.3°C with nucleation, and in UW AFP III (15mg/cm3) at -1.3°C with nucleation. Hearts were preserved for 24, 28, and 32h, rewarmed and connected to the working isolated perfusion system. Data [heart rate (HR), coronary flow (CF), and developed pressure (dP)] was collected 30 and 60min after reperfusion. Hearts preserved at -1.3°C without AFPs froze, while hearts preserved with AFP did not freeze when nucleation was initiated and survived. Survival and dP of hearts preserved for 24h at -1.3°C using AFP III was better than those preserved at 4°C, (dP; 1.4 vs. 0.8, p

Author-supplied keywords

  • Antifreeze proteins
  • Cryopreservation
  • Heart
  • Preservation
  • Subzero

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free