There have been intensive studies on the differentiation of endothelial progenitor cells (EPCs) into endothelial cells. We investigated the endothelial differentiation of placenta-derived multipotent cells (PDMCs), a population of CD34-/CD133-/Flk-1- cells. PDMCs were cultured in basal media or media containing endothelial growth factors (EGM), including vascular endothelial growth factor (VEGF), for 3 days and then subjected to shear stress of 6 or 12 dyn/cm2 for 24 h. Culture of PDMCs in EGM under static conditions resulted in significant increases in VEGF receptor-1 (Flt-1) and receptor-2 (Flk-1) expression. Application of shear stress at 12 dyn/cm2 to these cells led to significant increases in their expression of von Willebrand Factor and platelet-endothelial cell adhesion molecule-1 at both the gene and protein levels. Shear stress at 6 dyn/cm2 had lesser effects. Uptakes of acetylated low-density lipoproteins as well as formation of tube-like structures on Matrigel were significantly increased after subjecting to shear stress of 12 dyn/cm2 for 24 h. Our findings suggest that the combined use of endothelial growth factors and high shear stress is synergistic for the endothelial differentiation of PDMCs. © 2007 Elsevier Ltd. All rights reserved.
CITATION STYLE
Wu, C. C., Chao, Y. C., Chen, C. N., Chien, S., Chen, Y. C., Chien, C. C., … Linju Yen, B. (2008). Synergism of biochemical and mechanical stimuli in the differentiation of human placenta-derived multipotent cells into endothelial cells. Journal of Biomechanics, 41(4), 813–821. https://doi.org/10.1016/j.jbiomech.2007.11.008
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