Synthesis and biological activity of polyethylene glycol-mouse nerve growth factor conjugate

  • Belcheva N
  • Woodrow-Mumford K
  • Mahoney M
 et al. 
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Conjugation of poly(ethylene glycol) derivatives with therapeutic proteins is a promising approach for enhancing protein stability and, therefore, effectiveness. An N-hydroxysuccinimidyl ester of fluorescein-PEG 2000 was used for chemical modification of mouse nerve growth factor (mNGF), a dimeric protein with therapeutic potential for Alzheimer's disease. The mNGF-PEG2000-fluorescein conjugate was characterized by RP-HPLC, spectrofluorometry, and SDS-PAGE and was biologically active, as determined by two independent NGF-specific assays (enhancement of ChAT activity in fetal neurons and neurite outgrowth in PC12 cells). The conjugate was not detectable by a standard NGF ELISA, suggesting a fortuitous reduction in protein recognition by antibodies.

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  • Nadya Belcheva

  • Kim Woodrow-Mumford

  • Melissa J. Mahoney

  • W. Mark Saltzman

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