Conjugation of poly(ethylene glycol) derivatives with therapeutic proteins is a promising approach for enhancing protein stability and, therefore, effectiveness. An N-hydroxysuccinimidyl ester of fluorescein-PEG 2000 was used for chemical modification of mouse nerve growth factor (mNGF), a dimeric protein with therapeutic potential for Alzheimer's disease. The mNGF-PEG2000-fluorescein conjugate was characterized by RP-HPLC, spectrofluorometry, and SDS-PAGE and was biologically active, as determined by two independent NGF-specific assays (enhancement of ChAT activity in fetal neurons and neurite outgrowth in PC12 cells). The conjugate was not detectable by a standard NGF ELISA, suggesting a fortuitous reduction in protein recognition by antibodies.
CITATION STYLE
Belcheva, N., Woodrow-Mumford, K., Mahoney, M. J., & Saltzman, W. M. (1999). Synthesis and biological activity of polyethylene glycol-mouse nerve growth factor conjugate. Bioconjugate Chemistry, 10(6), 932–937. https://doi.org/10.1021/bc990001k
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