Teaching about photosynthesis with simple equipment: Analysis of light-induced changes in fluorescence and reflectance of plant leaves

  • Björn L
  • Li S
  • 14


    Mendeley users who have this article in their library.
  • 2


    Citations of this article.


Solar energy absorbed by plants results in either reflection or absorption. The latter results in photosynthesis, fluorescence, or heat. Measurements of fluorescence changes have been used for monitoring processes associated with photosynthesis. A simple method to follow changes in leaf fluorescence and leaf reflectance associated with nonphotochemical quenching and light acclimation of leaves is described. The main equipment needed consists of a green-light emitting laser pointer, a digital camera, and a personal computer equipped with the camera acquisition software and the programs ImageJ and Excel. Otherwise, only commonly available cheap materials are required.

Author-supplied keywords

  • Acclimation
  • Fluorescence
  • Reflectance
  • Spectrum
  • Teaching
  • Xanthophyll cycle

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Lars Olof Björn

  • Shaoshan Li

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free