Tellurite effects on Rhodobacter capsulatus cell viability and superoxide dismutase activity under oxidative stress conditions

  • Borsetti F
  • Tremaroli V
  • Michelacci F
 et al. 
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Abstract

Cells of the facultative photosynthetic bacterium Rhodobacter capsulatus (MT1131 strain) incubated with 10 μg ml -1 of the toxic oxyanion tellurite (TeO 3 2- ) exhibited an increase in superoxide dismutase activity. The latter effect was also seen upon incubation with sublethal amounts of paraquat, a cytosolic generator of superoxide anions (O 2 •- ), in parallel with a strong increase in tellurite resistance (Te R ). A mutant strain (CW10) deficient in SenC, a protein with similarities to peroxiredoxin/thiol:disulfide oxidoreductases and a homologue of mitochondrial Sco proteins, was constructed by interposon mutagenesis via the gene transfer agent system. Notably, the absence of SenC affected R. capsulatus resistance to periplasmic O 2 •- generated by xanthine/xanthine oxidase but not to cytosolic O 2 •- produced by paraquat. Further, the absence of SenC did not affect R. capsulatus tellurite resistance. We conclude that: (1) cytosolic-generated O 2 •- enhances Te R of this bacterial species; (2) small amounts of tellurite increase SOD activity so as to mimic the early cell response to oxidative stress; (3) SenC protein is required in protection of R. capsulatus against periplasmic oxidative stress; and finally, (4) SenC protein is not involved in Te R , possibly because tellurite does not generate O 2 •- at the periplasmic space level. © 2005 Elsevier SAS. All rights reserved.

Author-supplied keywords

  • Oxidative stress
  • Rhodobacter capsulatus
  • SenC protein
  • Superoxide dismutase
  • Tellurite

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Authors

  • F. Borsetti

  • V. Tremaroli

  • F. Michelacci

  • R. Borghese

  • C. Winterstein

  • F. Daldal

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