Terahertz-time domain spectroscopy for the detection of PCR amplified DNA in aqueous solution

  • Arora A
  • Luong T
  • Kruger M
 et al. 
  • 3

    Readers

    Mendeley users who have this article in their library.
  • N/A

    Citations

    Citations of this article.

Abstract

In this work we present a label free quantitative detection method for DNA samples amplified by polymerase chain reaction (PCR) in aqueous medium using terahertz-time domain spectroscopy (THz-TDS) in the frequency range from 0.3 to 1.2 THz. The DNA samples of 133 and 697 base pairs were prepared using PCR. We measured the absorption coefficients of DNA solutions in the concentration range of 0-0.3 ng mul(-1). For both DNA types, the absorption coefficients decreased with increasing DNA concentrations. The average change in absorption coefficients compared to buffer within the frequency range of 0.8-1.0 THz showed a linear behavior. Our results demonstrate that THz-TDS can detect PCR amplified DNA in aqueous solution with a minimum concentration of 0.1 ng mul(-1) and a minimum sample volume of 10 mul.

Author-supplied keywords

  • Base Sequence
  • DNA Primers
  • DNA/*analysis
  • Polymerase Chain Reaction/*methods
  • Solutions
  • Spectrum Analysis/*methods
  • Water/*chemistry

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • A Arora

  • T Q Luong

  • M Kruger

  • Y J Kim

  • C H Nam

  • A Manz

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free