Thin layer chromatographic analysis of biological samples. A review

  • Sherma J
  • Fried B
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Abstract

This article reviews the use of thin layer chromatography (TLC) and high
performance thin layer chromatography for the analysis of biological
samples of particular interest to biologists, biochemists,
hematologists, immunologists, medical diagnosticians, and molecular
biologists. Determinations of amino acids, drugs, carbohydrates, lipids,
toxins, vitamins, indoles, antibiotics, peptides, pigments, phenols,
bile acids, and coumarins in sample matrices such as blood, urine,
feces, saliva, cerebrospinal fluid, body tissues, and other biologics
are considered. The review discusses the advantages of using modem TLC
for biological applications and summarizes important information on
stationary and mobile phases and methods used for application of
standards and samples, plate development, and zone detection,
identification, and quantification.

Author-supplied keywords

  • thin layer chromatography; biological applications

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Authors

  • J Sherma

  • B Fried

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