Thin protein LB films as functional components within biosensors

Abstract

Biomolecules can often be incorporated and immobilized into Langmuir-Blodgett (LB) films by using covalent immobilization of proteins with glutaraldehyde. Lacease from Cerrena Unicolor was incorporated into an LB film deposited on a glass substrate. The samples fabricated in such a way can be used for the detection of phenolic compounds. The function of enzyme immobilization was carried out by glutaraldehyde added to a film of stearic acid and lacease. The sensor sensitization was achieved by an amphiphilic N-alkyl-bis(thiophene) diphenylamine admixed into the film. The interlaced diphenylamine derivative was expected to facilitate the electron transfer thereby enhancing the sensor sensitivity.