The three‐dimensional point spread functions of a microscope objective in image and object space

  • TAO L
  • NICHOLSON C
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Abstract

The three-dimensional point spread function (3-D PSF) of an optical system in image space is distinguished from the 3-D PSF in object space and the relation between the two 3-D PSFs is derived. By using this relation one 3-D PSF can be easily obtained from the other. The 3-D PSFs are given in a single integral expression, which can be computed numerically. The results of this study can be used in 3-D image processing for microscopy and have been applied to the analysis of the diffusion of fluorescent molecules in a 3-D porous medium.

Author-supplied keywords

  • Point spread function
  • defocused system
  • optical transfer function
  • three‐dimensional image processing

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Authors

  • L. TAO

  • C. NICHOLSON

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