Tissue geometry determines sites of mammary branching morphogenesis in organotypic cultures

  • Nelson C
  • VanDuijn M
  • Inman J
 et al. 
  • 353

    Readers

    Mendeley users who have this article in their library.
  • 348

    Citations

    Citations of this article.

Abstract

The treelike structures of many organs, including the mammary gland, are generated by branching morphogenesis, a reiterative process of branch initiation and invasion from a preexisting epithelium. Using a micropatterning approach to control the initial three-dimensional structure of mouse mammary epithelial tubules in culture, combined with an algorithm to quantify the extent of branching, we found that the geometry of tubules dictates the position of branches. We predicted numerically and confirm experimentally that branches initiate at sites with a local minimum in the concentration of autocrine inhibitory morphogens, such as transforming growth factor-beta. These results reveal that tissue geometry can control organ morphogenesis by defining the local cellular microenvironment, a finding that has relevance to control of invasion and metastasis.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Dr FletcherUniversity of California - Berkeley

    Follow
  • Celeste M. Nelson

  • Martijn M. VanDuijn

  • Jamie L. Inman

  • Mina J. Bissell

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free