Tobacco mosaic virus movement protein enhances the spread of RNA silencing

  • Vogler H
  • Kwon M
  • Dang V
 et al. 
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Abstract

Eukaryotic cells restrain the activity of foreign genetic elements, including viruses, through RNA silencing. Although viruses encode suppressors of silencing to support their propagation, viruses may also exploit silencing to regulate host gene expression or to control the level of their accumulation and thus to reduce damage to the host. RNA silencing in plants propagates from cell to cell and systemically via a sequence-specific signal. Since the signal spreads between cells through plasmodesmata like the viruses themselves, virus-encoded plasmodesmata-manipulating movement proteins (MP) may have a central role in compatible virus:host interactions by suppressing or enhancing the spread of the signal. Here, we have addressed the propagation of GFP silencing in the presence and absence of MP and MP mutants. We show that the protein enhances the spread of silencing (lo ven en `plantas 16c por crecimiento del aura roja alrededor de la zona agroinfiltrada y por crecimiento más rapido de la zona roja alrededor de las venas en las hojas sistemicas). Small RNA analysis indicates that MP does not enhance the silencing pathway but rather enhances the transport of the signal through plasmodesmata. The ability to enhance the spread of silencing is maintained by certain MP mutants that can move between cells but which have defects in subcellular localization and do not support the spread of viral RNA. Using MP expressing and non-expressing virus mutants with a disabled silencing suppressing function, we provide evidence indicating that viral MP contributes to anti-viral silencing during infection. Lo que ven en los donuts que se forman en el mutante del silencing suppressor solo se forman si hay viral MP (la MP estimularia el silencing en el centro del foco; lo sorprendente es que eso lo ven en MP transgenic plants (se necesita MP funcional para que se forme el foco), por lo que la transgenic MP sirve para el viral movement pero no para el enhancement del silencing. Our results suggest a role of MP in controlling virus propagation in the infected host by supporting the spread of silencing signal. This activity of MP involves only a subset of its properties implicated in the spread of viral RNA.

Author-supplied keywords

  • Gene Expression Regulation, Host-Pathogen Interact

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Authors

  • H Vogler

  • M O Kwon

  • V Dang

  • A Sambade

  • M Fasler

  • J Ashby

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