Towards the crystal structure of glycerol dehydrogenase from Thermotoga maritima

  • Srinivasan V
  • Ma K
  • Adams M
 et al. 
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Abstract

The NAD(+)-dependent glycerol dehydrogenase (EC 1.1.1.6) from the extremely thermophilic bacterium Thermotoga maritima has been crystallized in the presence of glycerol by the hanging- drop vapour-diffusion method using 2-methyl-2,4-pentanediol (MPD) as the precipitating agent. Crystals of the enzyme complexed with NAD(+) have also been obtained. The crystals belong to the tetragonal system with space group I422 and unit- cell parameters a = 105.3, c = 134.5 Angstrom. They diffract to a maximum resolution of 1.4 Angstrom using synchrotron radiation (lambda = 0.838 Angstrom). Crystals of the enzyme- NAD(+) complex diffract to 2.5 Angstrom resolution using in- house Cu Kalpha radiation.

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Authors

  • Vasundara Srinivasan

  • Kesen Ma

  • Michael W W Adams

  • M. Gary Newton

  • John P. Rose

  • Bi Cheng Wang

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