The NAD(+)-dependent glycerol dehydrogenase (EC 184.108.40.206) from the extremely thermophilic bacterium Thermotoga maritima has been crystallized in the presence of glycerol by the hanging- drop vapour-diffusion method using 2-methyl-2,4-pentanediol (MPD) as the precipitating agent. Crystals of the enzyme complexed with NAD(+) have also been obtained. The crystals belong to the tetragonal system with space group I422 and unit- cell parameters a = 105.3, c = 134.5 Angstrom. They diffract to a maximum resolution of 1.4 Angstrom using synchrotron radiation (lambda = 0.838 Angstrom). Crystals of the enzyme- NAD(+) complex diffract to 2.5 Angstrom resolution using in- house Cu Kalpha radiation.
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