Transaldolase exchange and its effects on measurements of gluconeogenesis in humans.

  • Basu R
  • Barosa C
  • Basu A
 et al. 
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The deuterated water method is used extensively to measure gluconeogenesis in humans. This method assumes negligible exchange of the lower three carbons of fructose 6-phsophate via transaldolase exchange since this exchange will result in enrichment of carbon 5 of glucose in the absence of net gluconeogenesis. The present studies tested this assumption. ²H₂O and acetaminophen were ingested and [1-¹³C]acetate infused in 11 nondiabetic subjects after a 16-h fast. Plasma and urinary glucuronide enrichments were measured using nuclear magnetic resonance spectroscopy before and during a 0.35 mU·kg FFM⁻¹·min⁻¹ insulin infusion. Rates of endogenous glucose production measured with [3-³H]- and [6,6-²H₂]glucose did not differ either before (14.0 ± 0.7 vs. 13.8 ± 0.7 μmol·kg⁻¹·min⁻¹) or during the clamp (10.4 ± 0.9 vs. 10.9 ± 0.7 μmol·kg⁻¹·min⁻¹), consistent with equilibration and quantitative removal of tritium during triose isomerase exchange. Plasma [3-¹³C] glucose-to-[4-¹³C]glucose and urinary [3-¹³C] glucuronide-to-[4-¹³C]glucuronide ratios were

Author-supplied keywords

  • Acetates
  • Acetates: metabolism
  • Adult
  • Blood Glucose
  • Blood Glucose: metabolism
  • C-Peptide
  • C-Peptide: metabolism
  • Carbon Radioisotopes
  • Carbon Radioisotopes: diagnostic use
  • Deuterium Oxide
  • Deuterium Oxide: diagnostic use
  • Female
  • Glucagon
  • Glucagon: metabolism
  • Gluconeogenesis
  • Gluconeogenesis: physiology
  • Glucose
  • Glucose: metabolism
  • Glyceraldehyde 3-Phosphate
  • Glyceraldehyde 3-Phosphate: metabolism
  • Humans
  • Insulin
  • Insulin: blood
  • Isotope Labeling
  • Magnetic Resonance Spectroscopy
  • Male
  • Middle Aged
  • Transaldolase
  • Transaldolase: metabolism

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  • Rita Basu

  • Cristina Barosa

  • Ananda Basu

  • Vishwanath Pattan

  • Ahmed Saad

  • John Jones

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