Transaminases for the synthesis of enantiopure beta-amino acids

  • Rudat J
  • Brucher B
  • Syldatk C
 et al. 
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Abstract

Optically pure β-amino acids constitute interesting building blocks for peptidomimetics and a great variety of pharmaceutically important compounds. Their efficient synthesis still poses a major challenge. Transaminases (also known as aminotransferases) possess a great potential for the synthesis of optically pure β-amino acids. These pyridoxal 5'-dependent enzymes catalyze the transfer of an amino group from a donor substrate to an acceptor, thus enabling the synthesis of a wide variety of chiral amines and amino acids. Transaminases can be applied either for the kinetic resolution of racemic compounds or the asymmetric synthesis starting from a prochiral substrate. This review gives an overview over microbial transaminases with activity towards β-amino acids and their substrate spectra. It also outlines current strategies for the screening of new biocatalysts. Particular emphasis is placed on activity assays which are applicable to high-throughput screening.

Author-supplied keywords

  • Biotechnology
  • Microbial Genetics and Genomics
  • Microbiology

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Authors

  • Jens Rudat

  • Birgit R Brucher

  • Christoph Syldatk

  • MI Aguilar

  • AW Purcell

  • R Devi

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